Recombinant:Article Title: Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Aves Labs Cat# GFP-1020; RRID:AB_10000240 Ki67 Abcam Cat# ab15580; RRID:AB_443209 Cleaved Caspase CST Cat# 9664s; RRID:AB_2070042 CD31 eBiosciencE Cat# 14-0311-82; RRID:AB_467201 CD31 Abcam Cat# ab28364; AB_726362 CD45 BioLegend Cat# 103101; RRID:AB_312966 CD3 Novusbio Cat# NB600-1441; RRID:AB_789102 P75 Millipore Cat# ab1554; RRID:AB_90760 Nestin Millipore Cat# MAB353; RRID:AB_94911 SOX10 Santa Cruz Cat# sc17342; RRID:AB_2195374 TYR Gift from Dr. Vincent Hearing, NCI N/A TRP1 Gift from Dr. Vincent Hearing, NCI N/A LYVE-1 eBioscience Cat# 14-0443-80; RRID:AB_1633416 VE-cadherin Abcam Cat# ab33168; RRID:AB_870662 S100 Sigma Cat# S2644; RRID:AB_477501 ICAM1 Abcam Cat# ab119871; RRID:AB_10900211 HMB45 Santa Cruz Cat# sc59305; RRID:AB_784471 alpha-SMA Abcam Cat# ab5694; RRID:AB_2223021 p-ERK Bioss Cat# bs-3016R; RRID:AB_10855752 p-p38 Bioss Cat# bs-2210R; RRID:AB_10856087 p21 Santa Cruz Cat# sc-6246; RRID:AB_628073 anti-chicken 488 Abcam Cat# ab150169; RRID:AB_2636803 anti-rabbit 594 Invitrogen Cat# A11012; RRID:AB_141359 anti-rabbit647 Abcam Cat# ab150075; RRID:AB_2752244 anti-rat 647 Abcam Cat# ab150159; RRID:AB_2566823 BRAFV600E Spring Bioscience clone VE1, E19290; AB_11203850 MITF Sigma Aldrich Cat# HPA003259; AB_1079381 CD31 DAKO Cat# M0823; AB_2114471 Chemicals, Peptides, and Recombinant Proteins Sunitinib Sigma PZ0012 4-Hydroxytamoxifen (4HT) Sigma H6278 Critical Commercial Assays Opal Polymer anti-Rabbit HRP Kit PerkinElmer N/A Deposited Data Single cell RNaseq data of human metastatic melanoma patient Tirosh et al., 2016 GEO: GSE72056 Single Cell RNaseq data from NRAS mouse model This study GEO: GSE148666 Experimental Models: Cell Lines PAECs ATCC PCS-100-022 YUWERA human melanoma cell Yale Skin Disease Research Core Center N/A Experimental Models: Organisms/Strains Mouse: K5-tTA;TRE-Edn3-lacZ FIU Animal Care Facility, Garcia et al., 2008 K5-Edn3 Mouse: Tg(Grm1)Epv(E) mice Gift from Dr. William Pavan, NIH Dct-Grm1 (Continued on next page) Cell Reports 31, 107765, June 16, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Dct-Grm1/K5-Edn3 This paper N/A Mouse: B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J The Jackson Lab TYR-CreERT2, # 012328 Mouse: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J The Jackson Lab mT/mG, #007576 Mouse: Dct-Grm1/K5-Edn3/TYR-CreERT2/mT/mG This paper N/A Mouse: Tyr::NRASQ61K/INK4a / KULeuven Animal Care Facility, Campagne et al., 2013 N/A Mouse: Dct-Grm1/K5-Edn3/mT/mG This paper N/A Mouse: Dct-Grm1/K5-Edn3/TYR-CreERT2 This paper N/A Software and Algorithms Fiji NIH and LOCI, University of Wisconsin https://imagej.net/Fiji GraphPad Prism GraphPad Software, La Jolla California USA https://www.graphpad.com Flowjo FlowJo, LLC, BD https://www.flowjo.com/
Polymer:Article Title: Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Aves Labs Cat# GFP-1020; RRID:AB_10000240 Ki67 Abcam Cat# ab15580; RRID:AB_443209 Cleaved Caspase CST Cat# 9664s; RRID:AB_2070042 CD31 eBiosciencE Cat# 14-0311-82; RRID:AB_467201 CD31 Abcam Cat# ab28364; AB_726362 CD45 BioLegend Cat# 103101; RRID:AB_312966 CD3 Novusbio Cat# NB600-1441; RRID:AB_789102 P75 Millipore Cat# ab1554; RRID:AB_90760 Nestin Millipore Cat# MAB353; RRID:AB_94911 SOX10 Santa Cruz Cat# sc17342; RRID:AB_2195374 TYR Gift from Dr. Vincent Hearing, NCI N/A TRP1 Gift from Dr. Vincent Hearing, NCI N/A LYVE-1 eBioscience Cat# 14-0443-80; RRID:AB_1633416 VE-cadherin Abcam Cat# ab33168; RRID:AB_870662 S100 Sigma Cat# S2644; RRID:AB_477501 ICAM1 Abcam Cat# ab119871; RRID:AB_10900211 HMB45 Santa Cruz Cat# sc59305; RRID:AB_784471 alpha-SMA Abcam Cat# ab5694; RRID:AB_2223021 p-ERK Bioss Cat# bs-3016R; RRID:AB_10855752 p-p38 Bioss Cat# bs-2210R; RRID:AB_10856087 p21 Santa Cruz Cat# sc-6246; RRID:AB_628073 anti-chicken 488 Abcam Cat# ab150169; RRID:AB_2636803 anti-rabbit 594 Invitrogen Cat# A11012; RRID:AB_141359 anti-rabbit647 Abcam Cat# ab150075; RRID:AB_2752244 anti-rat 647 Abcam Cat# ab150159; RRID:AB_2566823 BRAFV600E Spring Bioscience clone VE1, E19290; AB_11203850 MITF Sigma Aldrich Cat# HPA003259; AB_1079381 CD31 DAKO Cat# M0823; AB_2114471 Chemicals, Peptides, and Recombinant Proteins Sunitinib Sigma PZ0012 4-Hydroxytamoxifen (4HT) Sigma H6278 Critical Commercial Assays Opal Polymer anti-Rabbit HRP Kit PerkinElmer N/A Deposited Data Single cell RNaseq data of human metastatic melanoma patient Tirosh et al., 2016 GEO: GSE72056 Single Cell RNaseq data from NRAS mouse model This study GEO: GSE148666 Experimental Models: Cell Lines PAECs ATCC PCS-100-022 YUWERA human melanoma cell Yale Skin Disease Research Core Center N/A Experimental Models: Organisms/Strains Mouse: K5-tTA;TRE-Edn3-lacZ FIU Animal Care Facility, Garcia et al., 2008 K5-Edn3 Mouse: Tg(Grm1)Epv(E) mice Gift from Dr. William Pavan, NIH Dct-Grm1 (Continued on next page) Cell Reports 31, 107765, June 16, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Dct-Grm1/K5-Edn3 This paper N/A Mouse: B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J The Jackson Lab TYR-CreERT2, # 012328 Mouse: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J The Jackson Lab mT/mG, #007576 Mouse: Dct-Grm1/K5-Edn3/TYR-CreERT2/mT/mG This paper N/A Mouse: Tyr::NRASQ61K/INK4a / KULeuven Animal Care Facility, Campagne et al., 2013 N/A Mouse: Dct-Grm1/K5-Edn3/mT/mG This paper N/A Mouse: Dct-Grm1/K5-Edn3/TYR-CreERT2 This paper N/A Software and Algorithms Fiji NIH and LOCI, University of Wisconsin https://imagej.net/Fiji GraphPad Prism GraphPad Software, La Jolla California USA https://www.graphpad.com Flowjo FlowJo, LLC, BD https://www.flowjo.com/
Single Cell:Article Title: Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Aves Labs Cat# GFP-1020; RRID:AB_10000240 Ki67 Abcam Cat# ab15580; RRID:AB_443209 Cleaved Caspase CST Cat# 9664s; RRID:AB_2070042 CD31 eBiosciencE Cat# 14-0311-82; RRID:AB_467201 CD31 Abcam Cat# ab28364; AB_726362 CD45 BioLegend Cat# 103101; RRID:AB_312966 CD3 Novusbio Cat# NB600-1441; RRID:AB_789102 P75 Millipore Cat# ab1554; RRID:AB_90760 Nestin Millipore Cat# MAB353; RRID:AB_94911 SOX10 Santa Cruz Cat# sc17342; RRID:AB_2195374 TYR Gift from Dr. Vincent Hearing, NCI N/A TRP1 Gift from Dr. Vincent Hearing, NCI N/A LYVE-1 eBioscience Cat# 14-0443-80; RRID:AB_1633416 VE-cadherin Abcam Cat# ab33168; RRID:AB_870662 S100 Sigma Cat# S2644; RRID:AB_477501 ICAM1 Abcam Cat# ab119871; RRID:AB_10900211 HMB45 Santa Cruz Cat# sc59305; RRID:AB_784471 alpha-SMA Abcam Cat# ab5694; RRID:AB_2223021 p-ERK Bioss Cat# bs-3016R; RRID:AB_10855752 p-p38 Bioss Cat# bs-2210R; RRID:AB_10856087 p21 Santa Cruz Cat# sc-6246; RRID:AB_628073 anti-chicken 488 Abcam Cat# ab150169; RRID:AB_2636803 anti-rabbit 594 Invitrogen Cat# A11012; RRID:AB_141359 anti-rabbit647 Abcam Cat# ab150075; RRID:AB_2752244 anti-rat 647 Abcam Cat# ab150159; RRID:AB_2566823 BRAFV600E Spring Bioscience clone VE1, E19290; AB_11203850 MITF Sigma Aldrich Cat# HPA003259; AB_1079381 CD31 DAKO Cat# M0823; AB_2114471 Chemicals, Peptides, and Recombinant Proteins Sunitinib Sigma PZ0012 4-Hydroxytamoxifen (4HT) Sigma H6278 Critical Commercial Assays Opal Polymer anti-Rabbit HRP Kit PerkinElmer N/A Deposited Data Single cell RNaseq data of human metastatic melanoma patient Tirosh et al., 2016 GEO: GSE72056 Single Cell RNaseq data from NRAS mouse model This study GEO: GSE148666 Experimental Models: Cell Lines PAECs ATCC PCS-100-022 YUWERA human melanoma cell Yale Skin Disease Research Core Center N/A Experimental Models: Organisms/Strains Mouse: K5-tTA;TRE-Edn3-lacZ FIU Animal Care Facility, Garcia et al., 2008 K5-Edn3 Mouse: Tg(Grm1)Epv(E) mice Gift from Dr. William Pavan, NIH Dct-Grm1 (Continued on next page) Cell Reports 31, 107765, June 16, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Dct-Grm1/K5-Edn3 This paper N/A Mouse: B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J The Jackson Lab TYR-CreERT2, # 012328 Mouse: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J The Jackson Lab mT/mG, #007576 Mouse: Dct-Grm1/K5-Edn3/TYR-CreERT2/mT/mG This paper N/A Mouse: Tyr::NRASQ61K/INK4a / KULeuven Animal Care Facility, Campagne et al., 2013 N/A Mouse: Dct-Grm1/K5-Edn3/mT/mG This paper N/A Mouse: Dct-Grm1/K5-Edn3/TYR-CreERT2 This paper N/A Software and Algorithms Fiji NIH and LOCI, University of Wisconsin https://imagej.net/Fiji GraphPad Prism GraphPad Software, La Jolla California USA https://www.graphpad.com Flowjo FlowJo, LLC, BD https://www.flowjo.com/
RNA sequencing:Article Title: Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Aves Labs Cat# GFP-1020; RRID:AB_10000240 Ki67 Abcam Cat# ab15580; RRID:AB_443209 Cleaved Caspase CST Cat# 9664s; RRID:AB_2070042 CD31 eBiosciencE Cat# 14-0311-82; RRID:AB_467201 CD31 Abcam Cat# ab28364; AB_726362 CD45 BioLegend Cat# 103101; RRID:AB_312966 CD3 Novusbio Cat# NB600-1441; RRID:AB_789102 P75 Millipore Cat# ab1554; RRID:AB_90760 Nestin Millipore Cat# MAB353; RRID:AB_94911 SOX10 Santa Cruz Cat# sc17342; RRID:AB_2195374 TYR Gift from Dr. Vincent Hearing, NCI N/A TRP1 Gift from Dr. Vincent Hearing, NCI N/A LYVE-1 eBioscience Cat# 14-0443-80; RRID:AB_1633416 VE-cadherin Abcam Cat# ab33168; RRID:AB_870662 S100 Sigma Cat# S2644; RRID:AB_477501 ICAM1 Abcam Cat# ab119871; RRID:AB_10900211 HMB45 Santa Cruz Cat# sc59305; RRID:AB_784471 alpha-SMA Abcam Cat# ab5694; RRID:AB_2223021 p-ERK Bioss Cat# bs-3016R; RRID:AB_10855752 p-p38 Bioss Cat# bs-2210R; RRID:AB_10856087 p21 Santa Cruz Cat# sc-6246; RRID:AB_628073 anti-chicken 488 Abcam Cat# ab150169; RRID:AB_2636803 anti-rabbit 594 Invitrogen Cat# A11012; RRID:AB_141359 anti-rabbit647 Abcam Cat# ab150075; RRID:AB_2752244 anti-rat 647 Abcam Cat# ab150159; RRID:AB_2566823 BRAFV600E Spring Bioscience clone VE1, E19290; AB_11203850 MITF Sigma Aldrich Cat# HPA003259; AB_1079381 CD31 DAKO Cat# M0823; AB_2114471 Chemicals, Peptides, and Recombinant Proteins Sunitinib Sigma PZ0012 4-Hydroxytamoxifen (4HT) Sigma H6278 Critical Commercial Assays Opal Polymer anti-Rabbit HRP Kit PerkinElmer N/A Deposited Data Single cell RNaseq data of human metastatic melanoma patient Tirosh et al., 2016 GEO: GSE72056 Single Cell RNaseq data from NRAS mouse model This study GEO: GSE148666 Experimental Models: Cell Lines PAECs ATCC PCS-100-022 YUWERA human melanoma cell Yale Skin Disease Research Core Center N/A Experimental Models: Organisms/Strains Mouse: K5-tTA;TRE-Edn3-lacZ FIU Animal Care Facility, Garcia et al., 2008 K5-Edn3 Mouse: Tg(Grm1)Epv(E) mice Gift from Dr. William Pavan, NIH Dct-Grm1 (Continued on next page) Cell Reports 31, 107765, June 16, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Dct-Grm1/K5-Edn3 This paper N/A Mouse: B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J The Jackson Lab TYR-CreERT2, # 012328 Mouse: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J The Jackson Lab mT/mG, #007576 Mouse: Dct-Grm1/K5-Edn3/TYR-CreERT2/mT/mG This paper N/A Mouse: Tyr::NRASQ61K/INK4a / KULeuven Animal Care Facility, Campagne et al., 2013 N/A Mouse: Dct-Grm1/K5-Edn3/mT/mG This paper N/A Mouse: Dct-Grm1/K5-Edn3/TYR-CreERT2 This paper N/A Software and Algorithms Fiji NIH and LOCI, University of Wisconsin https://imagej.net/Fiji GraphPad Prism GraphPad Software, La Jolla California USA https://www.graphpad.com Flowjo FlowJo, LLC, BD https://www.flowjo.com/
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